Chromatography techniques, including thin layer chromatography (TLC), gas chromatography (GC), high-performance liquid chromatography (HPLC), and ion chromatography (IC), involve the use of a stationary phase and a mobile phase to separate the components of a mixture.
The rate of movement of the components is caused by the differences between the strengths of the interactions between atoms, molecules, or ions in the mobile and stationary phases.
- Predict the relative rates of movement of components along a stationary phase on the basis of their polarities and charge, given the structural formulae or relative polarities of the two phases.
Videos: Chromatography, Gas Chromatography (or alternative)
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Data from chromatography techniques can be used to determine the composition and purity of substances.
- Calculate and apply RF values and retention times in the identification of components in a mixture.
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Ion chromatography (also known as ion exchange chromatography) is used to remove either cations or anions from a mixture by replacing them with ions of another type.
- Explain, using equilibrium principles, how ions attached to the surface of a resin can be exchanged with ions in aqueous solution.